Aundre Jackson scored a game-high 23 points, and Cameron Satterwhite made four key free throws in the final 16 seconds as Loyola (Chicago) stunned No. 5 Florida 65-59 on Wednesday night in Gainesville, Fla.
Loyola beat a team ranked in the top five for the first time in 33 years.
Florida (5-3) has lost three consecutive games. The Gators fell to Duke 10 days ago before being upset by Florida State on Monday and then losing to Loyola (9-1).
Jackson, a senior, had a big night, hitting 10 of 12 shots from the field, including all three of his 3-point attempts. He came into the game as the team's top scorer and again led a balanced attack.
Freshman Cameron Krutwig helped with 14 points and eight rebounds. Donte Ingram added 11 points and eight rebounds. Satterwhite finished with only four points, but he got them at the right time.
The Ramblers had led most of the game before the Gators kept chipping away and cut their deficit to 61-59 in the final two minutes.
Satterwhite then made two free throws with 16 seconds left, extending the lead to four. He clinched the victory with two more free throws with one second left.
No Florida player reached double figures. Chris Chiozza and Jalen Hudson led the Gators with nine points apiece.
Florida shot just 36.9 percent from the field and missed 17 of 19 3-pointers. Loyola made 52 percent of its field-goal attempts and half of its 12 3-point tries.
Florida started shooting poorly for a second consecutive game, helping Loyola jump to some big early leads in the first half.
The Ramblers made 11 of their first 17 shots and rolled to a 27-14 lead midway through the first half before Florida slowly woke up on offense.
The Gators made a late run in the first half that cut the Ramblers' lead to 34-31 at halftime.
NOTES: This was the first meeting between the schools. ... Florida began the week averaging 99.5 points per game on offense, ranked first in the nation, but had scored 66 in a loss to Florida State on Monday before being held to 59 on Wednesday. ... The Ramblers now have won two straight after their only loss this season.